What is Chlorine Dioxide?
Antimicrobial Properties / Mode of Action
Chlorine dioxide (ClO2) acts as an oxidizing agent and reacts with several cellular constituents, including the cell membrane of microbes. By "stealing" electrons from them (oxidation), it breaks their molecular bonds, resulting in the death of the organism by the break up of the cell. Since chlorine dioxide alters the proteins involved in the structure of microorganisms, the enzymatic function is broken, causing very rapid bacterial kills. The potency of chlorine dioxide is attributable to the simultaneous, oxidative
attack on many proteins thereby preventing the cells from mutating to a resistant form. Additionally, because of the lower reactivity of chlorine dioxide, its antimicrobial action is retained longer in the presence of organic matter.
Sporal vs bacterial inactivation?
The difference between sporal and bacterial inactivation can be likened to the difference between sterilization and disinfection.
For example, for a chemical agent to be classified as a sterilant, it must be demonstrated to have sporicidal activity. For this reason, spores are commonly used as a challenge in sterilization process development. Disinfection, on the other hand, does not require the complete inactivation of microbial life and in some cases disinfectant claims may be substantiated by demonstrating bacterial inactivation capability.
The following is a simplistic explanation of the differences between a spore and a bacterium. Bacterial endospores are one of the most persistent forms of microbial life and typically require aggressive inactivation procedures. Vegetative bacteria are generally much more easily inactivated than are bacterial endospores. This is primarily because the sensitive areas of bacteria are easily contacted by chemosterilizing agents. The spore, however, has a more complex structure than the vegetative bacterial cell. Its sensitive material is contained within a core and that core is surrounded by a cortex and spore coats. These coats tend to act as a permeability barrier to the entry of chlorine dioxide and other compounds (Knapp).
CD Antimicrobial Spectrum of Activity:
Vegetative Bacteria: Bacterial Spores: Fungi:
• Staphylococcus aureus
• Bacillus subtilis *
• Aspergillus niger
• Pseudomonas aeruginosa
• Bacillus stearothermophilus
• Trychophyton mentagrophytes
• Salmonella cholerasuis
• Bacillus pumilus
• Candida albicans
• Mycobacterium smegmatis
• Clostridium sporogenes Viruses:
• Polio Type II (non-lipid)
• Herpes simplex Type I (lipid)
• Parvo Virus
(* CD Indicator Organism)
Found at:
www.clordisys.com/WhatIsCD.pdf